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SDS–PAGE for induced expression of recombinant proteins <t>(αA-crystallin-pet28,</t> βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.
Pet28 Expression Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet28 mhl expression vector  (Addgene inc)
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SDS–PAGE for induced expression of recombinant proteins <t>(αA-crystallin-pet28,</t> βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.
Pet28 Mhl Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet28 mhl expression vector - by Bioz Stars, 2026-03
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pet28 expression vector  (Millipore)
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Millipore pet28 expression vector
SDS–PAGE for induced expression of recombinant proteins <t>(αA-crystallin-pet28,</t> βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.
Pet28 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28 expression vector/product/Millipore
Average 90 stars, based on 1 article reviews
pet28 expression vector - by Bioz Stars, 2026-03
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pet28(+) expression vector  (Millipore)
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Millipore pet28(+) expression vector
SDS–PAGE for induced expression of recombinant proteins <t>(αA-crystallin-pet28,</t> βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.
Pet28(+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28(+) expression vector/product/Millipore
Average 90 stars, based on 1 article reviews
pet28(+) expression vector - by Bioz Stars, 2026-03
90/100 stars
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pet28 mbp tev 39 expression vector  (Addgene inc)
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Addgene inc pet28 mbp tev 39 expression vector
SDS–PAGE for induced expression of recombinant proteins <t>(αA-crystallin-pet28,</t> βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.
Pet28 Mbp Tev 39 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28 mbp tev 39 expression vector/product/Addgene inc
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pet28 mbp tev 39 expression vector - by Bioz Stars, 2026-03
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pet28 expression vector  (Qiagen)
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Qiagen pet28 expression vector
SDS–PAGE for induced expression of recombinant proteins <t>(αA-crystallin-pet28,</t> βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.
Pet28 Expression Vector, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28 expression vector/product/Qiagen
Average 90 stars, based on 1 article reviews
pet28 expression vector - by Bioz Stars, 2026-03
90/100 stars
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pet28(a+) expression vector  (Millipore)
90
Millipore pet28(a+) expression vector
SDS–PAGE for induced expression of recombinant proteins <t>(αA-crystallin-pet28,</t> βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.
Pet28(A+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28(a+) expression vector/product/Millipore
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pet28(a+) expression vector - by Bioz Stars, 2026-03
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SDS–PAGE for induced expression of recombinant proteins (αA-crystallin-pet28, βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.

Journal: Molecular Vision

Article Title: The expression of αA- and βB1-crystallin during normal development and regeneration, and proteomic analysis for the regenerating lens in Xenopus laevis

doi:

Figure Lengend Snippet: SDS–PAGE for induced expression of recombinant proteins (αA-crystallin-pet28, βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.

Article Snippet: The resulting 555 bp and 741 bp fragments were digested with Nco I and Hind III (Takara, Tokyo, Japan) at 37 °C for 5 h, cloned into pET28 expression vectors (Invitrogen) digested by Nco I and Hind III (Nco I and Psc I are isocaudarners).

Techniques: SDS Page, Expressing, Recombinant, Western Blot, Purification